Figure 5
From: LOC689986, a unique gene showing specific expression in restricted areas of the rodent neocortex
Immunohistochemistry analysis of the LOC689986 mouse ortholog in the developing and adult mouse brain. 20 μm sagittal cryosections from mice at three postnatal stages (P5, P10 and P30) were subjected to immunohistochemistry analysis. The fluorescent micrographs depict nuclei (DAPI), expressed LOC689986 mouse ortholog (LOC689986), 200 kD Neurofilament Heavy-chain (Neurofilament) and a merged image. A. Expression of the mouse ortholog (1700028K03Rik) in SCx of mice at P5 (a-d), P10 (e-h) and P30 (i-l). An abrupt border of protein expression observed between the SCx and the neighbouring MCx at P5 is indicated (d). B. Expression of the mouse ortholog (1700028K03Rik) in cerebellum of P5 (m-p), P10 (q-x) and P30 (y-af) mice. The mouse ortholog (1700028K03Rik) partially overlaps with the neurofilament marker at both P10 (t and x) and P30 (ab and af). The protein is apparently present in the cell body, nucleus and dendrites of Purkinje cells in the cerebellum (P10: t, and magnification: x, P30: ab, and magnification: af). Bar: 50 μm.