Figure 4
From: LOC689986, a unique gene showing specific expression in restricted areas of the rodent neocortex
LOC689986 protein expression analysis. A. The rabbit anti-LOC689986 antibody recognised a protein of approximately 25 kDa in tissue lysates from the temporal cortex, cingulate cortex, hippocampus and cerebellum. Gapdh immunodetection was used to examine equal protein loading. Ctrl: tissue lysate from a temporal cortex sample not subjected to anti-LOC689986 antibody. B. A protein of approximately 23 kDa was detected in transiently transfected HeLa cells, expressing a V5 tagged recombinant LOC689986 protein, using both an anti-V5 antibody (lane 1) and the anti-LOC689986 peptide antibody (lane 3). No recombinant protein was detected in mock-transfected cells (lane 2 and 4). No signal was detected in cell lysates from cells overexpressing the V5 tagged recombinant protein, or mock-transfected cells, when the rabbit anti-LOC689986 antibody was preabsorbed (lane 5 and 6, respectively). Actin immunodetection was used to check for equal protein loading.