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The 1st day 9:00
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Cage rocking (5 times per second) for 15 min
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The 2nd day 9:00
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Swimming in cold (4°C) water for 5 min
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The 3rd day 9:00
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Soiled cage (250 ml of tap water into the sawdust bedding) for 24 h
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The 4th day 9:00
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Lights on overnight
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The 5th day 9:00
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Cage tilting for 24 h
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The 6th day 9:00
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Restraint for 12 h
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The 7th day 8:00
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Food and water deprivation for 24 h
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The 8th day 8:00
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One-bottled-sucrose test sessions (last 1 h)
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The 8th day 9:00
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Soiled cage for 24 h
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The 9th day 9:00
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Electric stimulus (1.0 mA, each time for 1 second, 10 times per minute) for 5 min
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The 10th day 9:00
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Elevated temperature (40°C) for 15 min
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The 11th day 9:00
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Cage rocking for 15 min
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The 12th day 9:00
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Lights on overnight
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The 13th day 9:00
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Restraint for 12 h
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The 14th day 8:00
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Food and water deprivation for 24 h
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The 15th day 8:00
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One-bottled-sucrose test sessions (last 1 h)
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The 15th day 9:00
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Lights on overnight
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The 16th day 9:00
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Swimming in cold water for 5 min
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The 17th day 9:00
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Restraint for 12 h
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The 18th day 9:00
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Electric stimulus for 5 min
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The 19th day 9:00
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Cage rocking for 15 min
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The 20th day 9:00
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Soiled cage for 24 h
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The 21th day 9:00
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Cage tilting for 24 h (The CUS procedure finish)
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The 22th day 6:30
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Weighed body weight and open field test
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The 22th day 8:00
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Food and water deprivation for 24 h
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The 23th day 8:00
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One-bottled-sucrose test sessions (last 1 h)
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