Table 2 The CUS procedure
Time | Course of the experiment |
|---|---|
The 1st day 9:00 | Cage rocking (5 times per second) for 15Â min |
The 2nd day 9:00 | Swimming in cold (4°C) water for 5 min |
The 3rd day 9:00 | Soiled cage (250Â ml of tap water into the sawdust bedding) for 24Â h |
The 4th day 9:00 | Lights on overnight |
The 5th day 9:00 | Cage tilting for 24Â h |
The 6th day 9:00 | Restraint for 12Â h |
The 7th day 8:00 | Food and water deprivation for 24Â h |
The 8th day 8:00 | One-bottled-sucrose test sessions (last 1Â h) |
The 8th day 9:00 | Soiled cage for 24Â h |
The 9th day 9:00 | Electric stimulus (1.0Â mA, each time for 1Â second, 10 times per minute) for 5Â min |
The 10th day 9:00 | Elevated temperature (40°C) for 15 min |
The 11th day 9:00 | Cage rocking for 15Â min |
The 12th day 9:00 | Lights on overnight |
The 13th day 9:00 | Restraint for 12Â h |
The 14th day 8:00 | Food and water deprivation for 24Â h |
The 15th day 8:00 | One-bottled-sucrose test sessions (last 1Â h) |
The 15th day 9:00 | Lights on overnight |
The 16th day 9:00 | Swimming in cold water for 5Â min |
The 17th day 9:00 | Restraint for 12Â h |
The 18th day 9:00 | Electric stimulus for 5Â min |
The 19th day 9:00 | Cage rocking for 15Â min |
The 20th day 9:00 | Soiled cage for 24Â h |
The 21th day 9:00 | Cage tilting for 24Â h (The CUS procedure finish) |
The 22th day 6:30 | Weighed body weight and open field test |
The 22th day 8:00 | Food and water deprivation for 24Â h |
The 23th day 8:00 | One-bottled-sucrose test sessions (last 1Â h) |