Figure 2

Kainate-receptors can be activated by cortical afferents. A: Superimposed suprathreshold corticostriatal responses in a dSPN in control (red trace, control) and after adding 1 μM ACET to the superfusion (black trace, +ACET) to block KA-receptors while affecting, as less as possible, AMPA-receptors. After obtaining ACET-induced blockade, 10 μM CNQX were added to block remaining AMPA-receptors (pale grey trace, +CNQX). B: Superimposed suprathreshold responses to intrastriatal stimulation in a dSPN in control (red trace) and after adding ACET (black trace). After obtaining ACET-induced blockade, 10 μM CNQX were added to block AMPA-receptors (pale grey trace). C: Histogram summarizing similar results from a sample of experiments. D: Digital subtraction of the ACET-sensitive component from corticostriatal response disclosed a slowly rising and decaying response, while supplemental CNQX-sensitive fraction exhibited both fast early and slow late responses in dSPNs. E: Digital subtraction of ACET-sensitive constituent from response to intrastriatal stimulation mainly shows its contribution during PSP decay. Fast rising fraction is CNQX-sensitive. F: Superimposed suprathreshold corticostriatal responses in an iSPN in control (green trace) and after adding ACET to the superfusion (black trace). Subsequent application of CNQX shows additional AMPA-receptors blockade (pale grey trace, +CNQX). G: Superimposed suprathreshold responses to intrastriatal stimulation in an iSPN in control (green trace) and after adding ACET to the superfusion (black trace). Subsequent application of CNQX shows additional blockade (pale grey trace, +CNQX). H: Histogram representing similar results from a sample of experiments). I: Digital subtraction of ACET-sensitive component from corticostriatal response in iSPNs shows a slowly rising and decaying depolarization, while the CNQX-sensitive component shows fast early and late slow components. J: ACET-sensitive component to intrastriatal stimulation in iSPNs mainly shows its participation during the decaying phase of the PSP. CNQX-sensitivity includes the fast rising PSP.