D-Line mice present impaired motor coordination. The number of slips by male and female D-Line mice compared to non-transgenic littermates were measured with the challenging beam walk test in 6 (A), 9 (B) and 12 (C) months old individuals. Graphs show mean ± SEM; t-test. When analyzed by one way or two way ANOVA followed by Bonferroni’s post hoc test, factor transgene, no significant changes were observed. (A-C) D-Line male: n = 6; D-Line female: n = 6; wt male: n = 6; wt female: n = 6. (D) Pooled data of either sex on nest building behavior of D-Line mice compared to non-transgenic littermates in 9 months old individuals. Graph represents the mean nest building score (scoring from 1–5). D-Line (n = 8); ntg (n = 6). Graph shows mean ± SEM; Mann–Whitney U-test. * p < 0.05; ** p < 0.01.