Table 1 Antibodies used for immunocytochemical characterisation of cultured RBECs and RSCECs
Antigen | Species | Manufacturer | Concentration |
|---|---|---|---|
Caveolin 1 | Rabbit | Abcam ab2910 | 20 μg/ml |
Clathrin heavy chain | Rabbit | Abcam ab21679 | 20 μg/ml |
Claudin-5 | Mouse | Life Technologies 35-2500 | 10 μg/ml |
Occludin | Mouse | Life Technologies 33-1500 | 10 μg/ml |
P-gp | Mouse | Abcam ab3366 | 3.35 μg/ml |
Smooth muscle actin | Mouse | R & D Systems MAB1420 | 4 μg/ml |
Von Willebrand factor | Rabbit | Abcam ab6994 | 156 μg/ml |
ZO-1 | Rabbit | Abcam ab59720 | 10 μg/ml |