Figure 4
From: Hemopexin induces neuroprotection in the rat subjected to focal cerebral ischemia
HPX expression was evaluated in the ischemic penumbra at different time points following reperfusion. A, Western blotting of the HPX expression in sham and MCAO rats at 2 h, 12 h and 24 h after reperfusion. The expression of β-actin served as a loading control of the protein samples. The relative density of HPX protein to β-actin protein expression among groups was plotted. Data represent mean ± SEM. *P< 0.05 vs. Sham group. (n =4 for each group) Double immunofluorescence staining of HPX and NeuN/GFAP indicated that HPX expression in the cortex B, hippocampus C, and striatum D, were notably increased in the I/R group. Scale bars = 20 μm.