Table 3 Details of antibodies used for fluorescent immunohistochemistry
From: Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system
Primary antibodies | Species | Dilution | Company |
|---|---|---|---|
B0AT3 | Rabbit | 1:200 | Custom made (Innovagen, Sweden) |
B0AT3 | Mouse | 1:50 | Sigma-Aldrich, USA |
NeuN | Mouse | 1:400 | Millipore, Sweden |
MAP2 | Mouse | 1:500 | Sigma-Aldrich, USA |
Gad67 | Mouse | 1:200 | Millipore, Sweden |
VIAAT | Mouse | 1:300 | Synaptic Systems, Germany |
Synaptophysin | Mouse | 1:200 | BD Transduction lab, Sweden |
GFAP | Chicken | 1:400 | AbCam, United Kingdom |
Pan-cytokeratin | Mouse | 1:200 | Sigma-Aldrich, USA |
PAG | Mouse | 1:100 | AbCam, United Kingdom |
VGLUT1 | Guinea pig | 1:500 | Innovagen, Sweden, based on sequences described in [40, 67]. |
VGLUT2 | Guinea pig | 1:500 | Innovagen, Sweden, based on sequences described in [40, 67]. |
Secondary antibodies | Species | Dilution | Company |
Anti-rabbit-594 | Donkey | 1:400 | Invitrogen, USA |
Anti-guinea pig-488 | Goat | 1:400 | Invitrogen, USA |
Anti-mouse-488 | Goat | 1:400 | Invitrogen, USA |
Anti-chicken-488 | Goat | 1:400 | Invitrogen, USA |