Figure 6

B⁰AT3 immunoreactivity co-localized with neuronal and vesicle markers in primary cell cultures. Immunohistochemistry was performed on embryonic mouse E13 primary cell cultures with B⁰AT3 antibody (green), markers (red) and nucleus marker DAPI (blue). (A) Highly overlapping expression of B⁰AT3 and the neuronal marker MAP2 in the cell body close to the membrane and on dendrites in single cells from whole brain. Large nucleus belong to neuronal cells labelled with MAP2, while small nucleus represent glial cells. (B) Overlapping expression of B⁰AT3 and MAP2 in neuronal axons on cells from fore brain. (C) Co-localization of B⁰AT3 and the vesicular marker synapophysin on cells from fore brain (scale bar, 10 μm).