Figure 2

Progressive development of neuronal morphologies induced by RA treatment. M17 neuroblastoma cells were grown on cover slips. Cells were fixed, stained, and immunofluorescent images were taken (63X). β3-tubulin (red), synapsin-1/2 (green), and nuclei (blue). Undifferentiated M17 cells (A), development of radial glia-like morphology 72 h after RA addition (B), neurite extension and network formation (C, D).