Effect of CHF5074 and DAPT treatments on dendritic spine density and length in hippocampal pyramidal neurons. Low-power mag of CA1 hippocampal cortex (A) visualized by the Golgi-Cox technique; B-E: high-power mag (100x, 1.35NA) of spine morphology in hippocampal pyramidal neurons of wild-type (B), Tg2576 vehicle- (C), Tg2576 CHF5074 125 ppm- (D), CHF5074 375 ppm- (E) and DAPT 375 ppm- (F) treated mice. G. Quantification of the data, including those related to DAPT treatment, expressed as mean (± SEM) spine density and length of pyramidal neurons from the cerebral cortex of animals belonging to different experimental groups; the number of animals utilized for this analysis is indicated below the bars corresponding to each treatment group. *p < 0.05; **p < 0.01. Bars: A, 200 μm; B, 5 μm.