From: Method parameters’ impact on mortality and variability in rat stroke experiments: a meta-analysis
Regression formula for the effect of Strain and Type of middle cerebral artery occlusion procedure on Mortality rate (hypotheses 1B and 2B) | |||||
---|---|---|---|---|---|
Variable (reference category) | Variable categories | Regression coefficient | 0.95 confidence interval for regression coefficient | p-value | |
Constant | NA | 17.1 | 13.9 | 20.3 | 0.000 |
Strain (Sprague Dawley) | Wistar | 1.0 | −2.4 | 4.4 | 0.551 |
SHR | −6.9 | −12.8 | -.87 | 0.025 | |
WKY | −8.3 | −19.3 | 2.8 | 0.141 | |
Other Strains | 4.4 | −1.1 | 9.9 | 0.113 | |
Type of middle cerebral artery occlusion procedure (Intraluminal filament) | Direct, mechanical | −10.7 | −15.1 | −6.2 | 0.000 |
Embolic | 12.1 | 6.9 | 17.3 | 0.000 | |
Photothrombotic | −5.6 | −15.3 | 4.2 | 0.262 | |
Endothelin injection | −9.7 | −16.8 | −2.6 | 0.007 | |
Type of anesthetic (Inhalation anesthetic) | Chloral Hydrate | 4.1 | 0.17 | 8.1 | 0.041 |
Ketamine | 3.2 | −1.1 | 7.4 | 0.144 | |
Barbiturates and bensodiazepines | 1.1 | −4.6 | 6.8 | 0.710 | |
Other anesthetic | 3.0 | −1.9 | 8.0 | 0.230 | |
Awakening of rats during occlusion (No) | [Yes] | 9.1 | 3.9 | 14.2 | 0.001 |
Heart rate monitored (No) | [Yes] | −3.3 | −7.5 | 0.94 | 0.127 |
Blood glucose concentration analyzed (No) | [Yes] | −2.8 | −6.1 | 0.53 | 0.099 |
Type of infarct size evaluation (TTC) | Radiology | 5.4 | −0.11 | 10.9 | 0.055 |
Acidic/Basic stain or silver stain histology | −1.2 | −4.7 | 2.4 | 0.526 | |
Immunohistology | 10.4 | 3.2 | 17.6 | 0.005 | |
Criteria for excluding rats (None) | Observed absence of cerebral blood flow reduction | −4.0 | −6.9 | −1.1 | 0.008 |
Lack of functional deficit | 0.15 | −3.4 | 3.7 | 0.933 | |
Too small infarct | 0.22 | −5.1 | 5.6 | 0.935 | |
Other pathology in animal | −3.4 | −7.1 | 0.40 | 0.079 |