Figure 4
5-HT 1B and ET B receptor expression in cerebral arteries. 5-HT1B and ETB receptor expression and localisation was determined in middle cerebral artery sections by means of immunohistochemical stainings and confocal microscopy. Shown are representative photomicrographs for sham-operated rats (A and E), SAH rats with short acute CBF drop (B and F) and SAH rats with prolonged acute CBF drop (C and G). Upper row are photomicrographs showing green fluorescent staining with antibodies against the 5-HT1B receptor (A-C) and an example of a co-staining with antibodies against the 5-HT1B receptor (green fluorescence) and smooth muscle actin (red fluorescence) (D). Lower row are photomicrographs showing green fluorescent staining with antibodies against the ETB receptor (E-G) and an example of a co-staining with antibodies against the ETB receptor (green fluorescence) and smooth muscle actin (red fluorescence) (H). Two middle cerebral artery sections from each of 6 sham-operated rats and 8 SAH rats (4 from each acute CBF drop duration groups) were analyzed and one representative image for each group is shown.