Table 1 Antibodies used for Immunofluorescence staining
Antibody | Antigen | Host | Company | Catalog# | Dilution |
|---|---|---|---|---|---|
Primary antibody | |||||
NeuN | neuron | mouse | Abcam | ab104224 | 1:200 |
GFAP | astrocyte | mouse | Abcam | ab4648 | 1:200 |
Iba-1 | microglia | mouse | Abcam | ab15690 | 1:200 |
TNF-α | TNF-α | goat | Santa Cruz | SC-1351 | 1:50 |
Secondary antibody (conjugation) | |||||
Mouse IgG (Alexa Fluor 568) | Mouse IgG | goat | Invitrogen | A11031 | 1:400 |
Goat-IgG (DyLight 488) | Goat IgG | donkey | Abcam | ab96931 | 1:400 |