Figure 3

The effect of various concentrations of glucose on the cell cycle and apoptosis of NSCs following in vitro ischemia. (a) The cell cycle distribution of NSCs was analyzed by flow cytometry. The percentage of NSCs in G0/G1-phase was significantly increased, whereas the percentage of NSCs in S-phase was markedly decreased, compared with the control in all there higher glucose groups. (b) The apoptosis of NSCs was quantified by flow cytometry. Relative fluorescence in NSC populations was double-stained with Annexin V (AV)-FITC and propidium iodide (PI). In each panel, AV-/PI- cells are viable NSCs, shown in the lower-left quadrant. The AV+/PI + cells represent late apoptotic NSCs, shown in the upper-right quadrant, and the AV+/PI- cells represent early apoptotic NSCs, shown in the lower-right quadrant. AV-/PI + cells, representing necrotic NSCs, are shown in the upper-left quadrant. The proportion of apoptotic cells (both in early and late phase apoptosis) is higher in all the other three glucose groups compared with the control.