Sample | Reagent added | Total volume | Final concentrations | |||||
---|---|---|---|---|---|---|---|---|
 | Cytochrome c (200 μM) | Xanthine oxidase | Xanthine (80 μM) | MnTBAP (80 mg/L) | MPSS (120 mg/L) | ACSF |  |  |
 |  | (100 unit/L) |  |  |  |  |  |  |
Control | 100 μl | 100 μl | 100 μl | 0 | 0 | 100 μl | 400 μl | 50 μM cytochrome c, 25 unit/L xanthine oxidase, 20 μM xanthine |
MnTBAP-treated | 100 μl | 100 μl | 100 μl | 100 μl | 0 | 0 | 400 μl | 50 μM cytochrome c, 25 unit/L xanthine oxidase, 20 μM xanthine, 20 mg/L MnTBAP |
MPSS-treated | 100 μl | 100 μl | 100 μl | 0 | 100 μl | 0 | 400 μl | 50 μM cytochrome c, 25 unit/L xanthine oxidase, 20 μM xanthine, 30 mg/L MPSS |