Figure 6From: Selective modulation of chemical and electrical synapses of Helix neuronal networks during in vitro developmentEstimation of Helix C1 and B2 excitability. (A) Representative electrophysiological recordings from C1 and B2 neurons before and after 5-HT application. Both spontaneous activity (B, C) and the response to a depolarizing current step (0.5 nA for 10 seconds; D, E) were measured. (B) B2 neurons showed a significant increase of spontaneous firing activity only after 5-HT treatment. At 24 hours after plating no activity was observed. Conversely, C1 neurons (C) were silent in all the experimental groups. During the electrical stimulation, B2 exhibited a similar firing rate (D), while a higher firing frequency was evoked in C1 neurons only after 5-HT application. (E) Comparable values were recorded 24 hours after plating.Back to article page