Antibody characterization (A-C) and overall laminar distribution of RIP3 immunolabeling in the adult rat retina (D-F). Panel (A) shows an immunoblotting test of the antibody, which reveals a single band migrated at 57 kDa, blockable by preincubation of the immunogenic peptide with primary antibody. Panels (B and C) show that the antibody labeling (B) can also be eliminated by the pre-absorption peptide in immunofluorescent preparation. Panels (D) and enlargements (E, F) show RIP3 immunolabeling revealed by the avidin-biotin-peroxidase method. Labeling appears mostly in the inner retinal layers and the pigment epithelium layer (PEL) (D). Many labeled somata are present in the ganglion cell layer and some also seen in the inner nuclear layer (INL). PL: photoreceptor layer; ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer; NFL, nerve fiber layer. Scale bar=70 μm in B, C and 100 μm in D, 50 μm in E, F.