Figure 6

DRG neurons express LN. Dissociated DRG neurons were immunostained for laminin (red), neuron-specific βIII tubulin (green) and IB4 lectin labeling (blue) at t=0 (A-D) and t=24 hr after plating on poly-lysine (E-H). Neurons cultured on poly-lysine were used to detect the LN expression, as it was not possible to detect the cellular LN signal above the stained LN substrate. ICC quantitation showed that IB4⁺ cells had lower levels of LN expression at t=0 compared to the IB4^- cells. Scale bar-20 μm.