Figure 1

IB4⁺DRG neurons do not respond to a LN substrate with neurite growth. DRG neurons were separated using isolectin B4 (IB4) coated magnetic beads (as described in the Methods) to produce two populations of neurons - IB4 selected (IB4⁺) and IB4^-. Each cell group was plated on PL (A, C) and LN (B, D) coated culture dishes for 24 hr at 37°C. Cells were immunostained for βIII tubulin (green), integrin β1 (red) expression and IB4 lectin binding (blue) labeling. Note that while the larger IB4- neurons show extensive neurite growth, the IB4⁺ cells have no detectable growth. Scale bar-20 μm.