TEM confirmation of neuronal and glial apoptosis. The tissue for TEM was obtained at 24 h post-SCI from layer VIII of the ventral horn of the cord and the 2–2.5 mm rostral from the epicenter were processed for TEM as described in the Methods. A: a normal neuron from sham control (×14,300). B: a degenerating neuronal axon (×3,300). C (×24,475); D (×14,300); E (×42,625); F (×18,150): different stages of apoptotic neurons and glial cells. Symbols: N-Nucleus, M-Mitochondria, R- Rough endoplasmic reticulum, G-Golgi complex, Ch-Chromatin.