Figure 3

Acute increases in cell proliferation in the subventricular zone (SVZ) of the cerebral hemisphere ipsilateral to a CCI. (a) Short-term BrdU incorporation analysis identifies proliferating cells, including mitotically active neural stem cells, in the SVZ lining the lateral wall of the forebrain lateral ventricle. Mice were processed for anti-BrdU IHC at 74 hours post-procedure following BrdU at 0, 24, 48 and 72 hours post-procedure. The images were taken from the SVZ of coronal sections of the cerebral hemisphere ipsilateral to the CCI/sham. Key: CC, corpus callosum; LV, lateral ventricle; Str, striatum. Scale bar = 20 μm. (b) Stereological cell counts reveal a significant increase in the number of BrdU-incorporated cells in the SVZ of the cerebral hemisphere ipsilateral but not contralateral to a moderate CCI (* p < 0.05 moderate CCI versus naïve [n = 4/group], one-way ANOVA with Tukey’s multiple comparison post test). Although the data reveals a trend of increasing BrdU + cell numbers in both SVZs with increasing injury severity, no other increases were significant when compared to naïve.