Figure 5

Immunocytochemical analysis of Ca²⁺-activated K⁺channel and ET _A R expressions in Ntg and GET-1 MCA after SAH. (A) Representative micrograph shows the localization and expression of Ca²⁺-activated K⁺ channel in the MCA of the Ntg and GET-1 after SAH. (n = 3 from each group of mice). Histogram shows the quantification results (relative value in percentage) of immunocytochemistry of sham and SAH of Ntg and GET-1 MCA sections. (*P < 0.05, **P < 0.01, ***P < 0.005, ANOVA followed by Bonferroni’s test; n = 3 for each group of mice). (B) Representative micrograph shows the localization and expression of ET_AR in the smooth muscle cells (arrows) of MCA in Ntg and GET-1 mice. Histogram shows the quantification results (relative value in percentage) of immunocytochemistry of sham and SAH of Ntg and GET-1 MCA sections. (*P < 0.05, **P < 0.01, ANOVA followed by Bonferroni’s test; n = 3 for each group of mice). (C) Representative micrograph shows the immunocytochemical expressions of PKC-α and the diameters of MCA after treating with ET_AR antagonist ABT-627. Histogram below shows the measurements of MCA diameter. (*P < 0.05, **P < 0.01, Mann-Whitey test; n = 3 for Ntg and GET-1 mice).