Figure 2

GET-1 mice showed more severe brain edema with upregulation of ET-1 and GFAP expressions after subarachnoid hemorrhage. (A) Analysis the integrity of the BBB with the EB extravasation in Ntg and GET-1 brain after SAH. (*P < 0.05, ANOVA followed by Bonferroni’s test; n = 4 for the sham groups and n = 8 for the SAH groups). (B) Histogram comparing the water content of the Ntg and GET-1 mice with or without SR 49059 treatment after SAH (*P < 0.05, ANOVA followed by Bonferroni’s test; n = 4 for each group of mice). (C) Immunocytochemical analysis of ET-1 expression in Ntg brain after SAH. Representative micrograph shows the expression of ET-1 in the hippocampal region of the Ntg brain after Sham (i) and SAH (ii) (n = 4 for each group). Histogram below shows the quantification results (relative value in percentage) of ET-1 Sham and SAH of Ntg brain sections using software ImageJ. (**P < 0.01, student t-test; n = 4 for each group of mice). (D) Immunocytochemical analysis of GFAP expression after SAH. Representative micrograph shows the localization and expression of GFAP in the astrocytes of the hippocampus of the Ntg and GET-1 brain at (i-iv) low and (v-viii) high magnification (n = 4 for each group). The GFAP signals in the astrocytes at the SAH groups of the Ntg and GET-1 brains is increased, and they have longer processes (arrowheads). Histogram below shows the quantification results (relative value in percentage) of GFAP Sham and SAH of Ntg and GET-1 brain sections using software ImageJ. (*P < 0.05, **P < 0.01, ***P < 0.005, ANOVA followed by Bonferroni’s test; n = 4 for each group of mice).