Cells in CE neurospheres activate RGC-specific regulatory gene expression. CE neurospheres were cultured in the presence of E14CM for 5 days and RGC regulatory gene expression  was examined (A). Q-PCR analysis of cells revealed a significant increase in levels of transcripts corresponding to the regulators of differentiation (Atoh7 and Brn3b) and maturation (Rpf1, Thy1, and Sncg) of RGCs in differentiation conditions, compared to controls (B). However, no significant induction in the expression of Isl1 was observed. Controls: CE untreated cells.