Examination of the effect of astrocyte conditioned media on interneurons derived from differentiation of mouse embryonic stem cells. (a) Double-labelled fluorescence image of culture grown in wt non-Tg astrocyte CM labelled for Lhx1/5 interneurons (red), Hb9:eGFP MNs (green) and DAPI for nuclei (blue). Scale bar 50 μm. (b) Analysis of the number of interneurons in control medium, wt non-Tg and Tg SOD1G93A CM. Data gathered from 2 independent experiments, each condition examined in triplicate and analysed by one-way ANOVA followed by Tukey’s post-test. No significant differences in the proportion of interneurons were found between the three media.