Figure 5

Effect of delaying UAS-dNSF2^E/Qon synaptic physiology. EJP amplitudes were measured from larvae initially reared at 25°C (Gal4 off) and then shifted to 30°C (Gal4 on). If expression is only delayed for 1 or 2 days, EJP amplitudes are small and comparable to full continuous UAS-dNSF2^E/Q expression. If expression is delayed by 3 days or more, the EJP amplitudes return to normal levels. The bars represent mean ± SEM EJP amplitude (mV) with the number of muscles recorded indicated within each bar. Letters within the bars represent statistically similar groups determined with ANOVA (p < 0.05) and the Dunnett post hoc comparison of all conditions to full UAS-dNSF2^E/Q expression.