Neurological behavior score. The Garcia Test was applied to determine the neurological deficit in the rats receiving ischemia–reperfusion injury by MCAO in animals receiving different doses of E2. The OVX group demonstrated significantly lower neurological deficit scores than the control group. Treatment with 6 μg/kg or 20 μg/kg E2 significantly improved the neurological deficit scores. Treatment with 50 μg/kg E2 resulted in significantly lower neurological deficit scores (*p < 0.01 v.s. the control group, # p < 0.05 v.s. the OVX group).