Figure 4

Fluorescence microscopy of astroglial and oligodendroglial lineage cells derived from dividing cells. Panel A is a confocal photomicrograph that shows an S100β+ (red) astroglial cell in the cortex of a GFP-global line mouse brain. Panel B is a confocal photomicrograph that shows BrdU+ (green) cells in the same field. Panel C represents the merging of panels A and B and indicates the cell (arrow) is a double positive S100β+/BrdU+ (yellow) astroglial cell, which has undergone cell division. Scale bar in panels A, B and C represents 15 μm. Panel D is a confocal photomicrograph showing Olig2+ (red) cells in the striatum of a mutant mouse brain from the GFP-global line. Panel E is a confocal photomicrograph that shows BrdU+ (green) cells in the same field. Panel F represents the merging of panels D and E and indicates that two cells (arrows) are Olig2+/BrdU+ (yellow) and are therefore oligodendroglial lineage cells, which have undergone cell division. Scale bar in panels D, E and F represents 12 μm.