Figure 3

Regional quantification of neuronal, microglial, astroglial and oligodendroglial lineage cells derived from dividing progenitors in GFP-control and GFP-global mutant mice. Graphs showing the number of double-positive NeuN+/BrdU+, Iba1+/BrdU+, S100β+/BrdU+ and Olig2+/BrdU+ cells at specific sites relative to bregma in the M1 motor cortex (A-D), striatum (E-H) and periventricular (I-L) regions of the brain of mice belonging to the GFP-global line and GFP-control mice injected for 2 weeks with BrdU+ at 4 weeks of age and killed at age 8 weeks of age for analysis. Each point represents the mean ± SEM as determined for each bregma level in mutant (n=4) and control (n=4) mice. There was a greater number of NeuN+/BrdU+ cells in GFP-global mutant relative to control mice measured at 0.26mm from bregma (**P<0.01) as determined by a Bonferroni post-hoc test. There was also a significant increase in the number of NeuN+/BrdU+ cells in the striatum (P< 0.01) and periventricular regions (P< 0.01), Iba1+/BrdU+ cells in all three regions (all P< 0.01), S100β+/BrdU+ cells in the cortex (P< 0.01) and periventricular regions (P< 0.01) and Olig2+/BrdU+ cells in the cortex (P< 0.01) and striatum (P< 0.05) in GFP-global mutant relative to control mice.