Figure 1

Construct expression and processing. In the construct of ETNA Aβ(3–42) is fused to pre-pro-TRH for product liberation within the secretory pathway. After prohormone convertase cleavage the N-terminally truncated Aβ peptide is transported into the trans-Golgi and secretory vesicles, where the N-terminus is available to QC for cyclization (A); adapted from Alexandru et al., 2011. Quantification of protein levels by ELISA of ETNA brains revealed highest total Aβ levels for hom E8, lowest for hom E5 and intermediate for het/het E85 at two different ages (B); total Aβ values expressed as percentage of hom E8 at the age of 9 months; data represent means ± SEM; n ≥ 5 animals per genotype. These protein level relations between the three lines are reflected by similar relations of pE3-Aβ levels at the age of 9 months (C); data represent means ± SEM; n ≥ 5 animals per genotype.