Table 1 Composition of cell culture media to expand the STROC05 cell line
Component | Concentration | Supplier |
|---|---|---|
DMEM: F:12 | Base media | Gibco |
Human albumin solution | 0.03% | Baxter |
Human insulin | 5 μg/ml | Sigma |
L-glutamine | 2 mM | Sigma |
Putrescine DiHCl | 16.2 μg/ml | Sigma |
Sodium Selenite | 40 ng/ml | Sigma |
L-Thyroxine (T4) | 400 ng/ml | Sigma |
Tri-iodo-thyronine (T3) | 337 ng/ml | Sigma |
Progesterone | 60 ng/ml | Sigma |
Corticosterone | 20 μg/ml | Sigma |
*bFGF-2 | 10 μg/ml | PeproTch |
*EGF | 10 μg/ml | Invitrogen |
*4-OHT-tamoxifen | 100nM/ml | Sigma |