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Table 2 Intra- and inter-assay imprecision in the r-sAPPα assay

From: New highly sensitive rodent and human tests for soluble amyloid precursor protein alpha quantification: preclinical and clinical applications in Alzheimer’s disease

Intra-assay imprecision

Neuron conditioned medium

r-sAPPα (ng/ml)

CV (%)

1

23.4 ± 1.4

5.9

2

15.9 ± 0.7

4.4

3

17.4 ± 0.6

3.4

Inter-assay imprecision

Neuron conditioned medium

r-sAPPα (ng/ml)

CV (%)

1

24.7 ± 1.5

6.0

2

14.8 ± 1.7

11.4

3

18.6 ± 1.2

6.4

  1. Intra-assay imprecision: three conditioned media samples from three different experiments were tested ten times on one plate.
  2. Inter-assay imprecision: the same samples were tested three times in three independent assays. Mean ± SEM of r-sAPPα concentration (ng/ml). CV: coefficient of variation (%).