Figure 8

Representative data showing that doublecortin-immunoreactive (DCX+) cells around neocortical layer II in Pb-treated animals surviving 6 months do not colocalize with BrdU (A-C), active caspase-3 (D-G) or Fluoro-Jade C labeling (H-J). Markers and fluorescent channels are indicated on the left of each panel. Note that BrdU labeled cells (arrows) are present in layer I and upper III (A, C). Nuclei labeled for active caspase-3 are occasionally seen in layer I (D, F, and G). Fluoro-Jade C labeled cells are rarely detected in the cortex (H-J) or normal rat retina (N-P). In contrast, they are found in the rat retina 24 h after intraocular hypotension (K-M). PL: photoreceptor layer; ONL: outer nuclear layer; EPL: external plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer. Scale bar = 100 μm in A applying to D, H, I, K, L, N and O; equal to 50 μm for B, C, E-G, J, M and P.