Selenium pretreatment alters autophagy activation following cerebral ischemia. A. Representative Western blot and analysis of autophagy markers Beclin 1 and LC3 shows that selenium pretreatment lowers that intensity Beclin 1 and LC3-II as compared to saline treatment. B. Immunolocalization of LC3 (green) and pDrp1 (red) indicates that cerebral ischemia leads to conversion of LC3-I (diffused) to LC3-II (punctated). Punctated LC3 pattern overlaps with pDrp1 staining. Selenium pretreatment, on contrary, decreases LC3-II (punctated) staining. The western results were normalized as relative % of control set at 100% (n = 4 each group). Data represents mean ± SD. *p < 0.05, and ***P < 0.001 vs control and ##p < 0.01, ###p < 0.001 vs. respective time point. Se = selenium and Cont = control. Bar = 100 μm.