Selenium pretreatment increases protein levels of mitochondrial biogenesis markers, PGC-1α and NRF1. Representative Western blot and analysis of PGC-1α and NRF1 in control, 5- and 24 h of recirculation in saline and selenium pretreated groups (n = 4 each group). The results were normalized as relative % of control set at 100%. Data represents mean ± SD. *p < 0.05, and ***p < 0.001 vs control and ##p < 0.01, ###p < 0.001 vs. respective time point. Se = selenium and Cont = control.