Neuroprotective effect of selenium pretreatment against glutamate toxicity and hypoxia. A. Effect of selenium pretreatment on glutamate toxicity and hypoxic cell death. Cell viability was measured 24 h or 10/12 h after glutamate and hypoxia/reoxygenation respectively. Selenium pretreatment significantly improved cell survival from glutamate toxicity (4 mM) and hypoxia/reoxygenation (10/12 h). B. Representative micrograph of HT22 cells exposed to glutamate (4 mM) and hypoxia/reoxygenation (10/12 h) with or without selenium pretreatment. Cell viability was calculated using MTT assay and relative cell viability was expressed as % of control set at 100%. Data represents means ± SD of 4 experiments conducted in triplicate. *P < 0.05, ***P < 0.001 vs. control and #p < 0.05, ###p < 0.001 vs. respective group. Se = selenium, Cont = control and Glu = glutamate.