Figure 2

MMPSense750 activation at acute (2 hour) and subacute (24 hour) time points after reperfusion from MCAO. MMPSense750 injection in sham animals (A) resulted in no significant ipsilateral versus contralateral differences in fluorescent intensity whereas with acute MCAO reperfusion (B), there were significant differences in fluorescent intensity by 30 minutes (P <0.05) post injection that persisted until 180 minutes. At 24 hours post ischemia there were immediate ipsilateral-contralateral differences following probe injection that persisted for all the times examined. The increase in ipsilateral signal intensity was attenuated by hypothermia (D). * P <0.05, ** p <0.01 comparison to pre-injection value (One way repeated measures ANOVA, non parametric Dunnet post test).