|
Subunit combination
|
[125I]-α-BTX Binding
|
[3 H]-epibatidine binding
|
|---|
| |
- RIC3
|
+ RIC3
|
- RIC3
|
+ RIC3
|
|
Dα5
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα4
|
–
|
–
|
–
|
–
|
|
Dα5/Dα6
|
–
|
+
|
–
|
–
|
|
Dα5/Dα7
|
–
|
–
|
–
|
–
|
|
Dα5/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα3/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα3/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα3/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα4/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα4/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα4/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα6/Dα7
|
–
|
–
|
–
|
–
|
|
Dα5/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dβ1/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dβ2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα3/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα3/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα3/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα4/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα4/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα4/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dα3/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dα3/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dα3/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dα4/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dα4/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dα4/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα3/Dα4/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα3/Dα4/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα3/Dα4/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα6/Dα7/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα6/Dα7/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα6/Dα7/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dβ1/Dβ2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dα3/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dα3/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dα3/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα3/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα4/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dα3/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dα4/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα3/Dα4/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dβ2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα3/Dβ2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα4/Dβ2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dβ1/Dβ2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dα3/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dα4/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα2/Dβ1/Dβ2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα3/Dα4/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα3/Dβ1/Dβ2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dα3/Dα4/Dβ1
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dα3/Dα4/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dα3/Dα4/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα6/Dα7/Dβ1/Dβ2/Dβ3
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dα3/Dα4/Dα6/Dα7/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dα3/Dα4/Dα6/Dα7/Dβ1/Dβ2
|
–
|
–
|
–
|
–
|
|
Dα5/Dα1/Dα2/Dα3/Dα4/Dα6/Dα7/Dβ1/Dβ2/Dβ3
|
–
|
–
|
–
|
–
|
- To illustrate the extent of radioligand binding studies undertaken, the Table lists all subunit combinations containing Dα5 that were examined in transfected Drosophila S2 cells. Binding studies were performed with [125I]-α-bungarotoxin (10 nM) and [3 H]-epibatidine (30 nM). Combinations of Drosophila nAChR subunit cDNAs were transfected in the absence or presence of RIC-3 cDNA. Data indicating presence or absence of specific binding are derived from at least 3 independent experiments.
