Skip to main content

Table 1 Radioligand binding to  Drosophila  nAChR subunit combinations

From: The Drosophila nicotinic acetylcholine receptor subunits Dα5 and Dα7 form functional homomeric and heteromeric ion channels

Subunit combination

[125I]-α-BTX Binding

[3 H]-epibatidine binding

 

- RIC3

+ RIC3

- RIC3

+ RIC3

Dα5

–

–

–

–

Dα5/Dα1

–

–

–

–

Dα5/Dα2

–

–

–

–

Dα5/Dα3

–

–

–

–

Dα5/Dα4

–

–

–

–

Dα5/Dα6

–

+

–

–

Dα5/Dα7

–

–

–

–

Dα5/Dβ1

–

–

–

–

Dα5/Dβ2

–

–

–

–

Dα5/Dβ3

–

–

–

–

Dα5/Dα1/Dα2

–

–

–

–

Dα5/Dα1/Dβ1

–

–

–

–

Dα5/Dα1/Dβ2

–

–

–

–

Dα5/Dα1/Dβ3

–

–

–

–

Dα5/Dα2/Dβ1

–

–

–

–

Dα5/Dα2/Dβ2

–

–

–

–

Dα5/Dα2/Dβ3

–

–

–

–

Dα5/Dα3/Dβ1

–

–

–

–

Dα5/Dα3/Dβ2

–

–

–

–

Dα5/Dα3/Dβ3

–

–

–

–

Dα5/Dα4/Dβ1

–

–

–

–

Dα5/Dα4/Dβ2

–

–

–

–

Dα5/Dα4/Dβ3

–

–

–

–

Dα5/Dα6/Dα7

–

–

–

–

Dα5/Dβ1/Dβ2

–

–

–

–

Dα5/Dβ1/Dβ3

–

–

–

–

Dα5/Dβ2/Dβ3

–

–

–

–

Dα5/Dα1/Dα2/Dβ1

–

–

–

–

Dα5/Dα1/Dα2/Dβ2

–

–

–

–

Dα5/Dα1/Dα2/Dβ3

–

–

–

–

Dα5/Dα1/Dα3/Dβ1

–

–

–

–

Dα5/Dα1/Dα3/Dβ2

–

–

–

–

Dα5/Dα1/Dα3/Dβ3

–

–

–

–

Dα5/Dα1/Dα4/Dβ1

–

–

–

–

Dα5/Dα1/Dα4/Dβ2

–

–

–

–

Dα5/Dα1/Dα4/Dβ3

–

–

–

–

Dα5/Dα2/Dα3/Dβ1

–

–

–

–

Dα5/Dα2/Dα3/Dβ2

–

–

–

–

Dα5/Dα2/Dα3/Dβ3

–

–

–

–

Dα5/Dα2/Dα4/Dβ1

–

–

–

–

Dα5/Dα2/Dα4/Dβ2

–

–

–

–

Dα5/Dα2/Dα4/Dβ3

–

–

–

–

Dα5/Dα3/Dα4/Dβ1

–

–

–

–

Dα5/Dα3/Dα4/Dβ2

–

–

–

–

Dα5/Dα3/Dα4/Dβ3

–

–

–

–

Dα5/Dα6/Dα7/Dβ1

–

–

–

–

Dα5/Dα6/Dα7/Dβ2

–

–

–

–

Dα5/Dα6/Dα7/Dβ3

–

–

–

–

Dα5/Dβ1/Dβ2/Dβ3

–

–

–

–

Dα5/Dα1/Dα2/Dα3/Dβ1

–

–

–

–

Dα5/Dα1/Dα2/Dα3/Dβ2

–

–

–

–

Dα5/Dα1/Dα2/Dα3/Dβ3

–

–

–

–

Dα5/Dα1/Dα2/Dβ1/Dβ2

–

–

–

–

Dα5/Dα1/Dα3/Dβ1/Dβ2

–

–

–

–

Dα5/Dα1/Dα4/Dβ1/Dβ2

–

–

–

–

Dα5/Dα2/Dα3/Dβ1/Dβ2

–

–

–

–

Dα5/Dα2/Dα4/Dβ1/Dβ2

–

–

–

–

Dα5/Dα3/Dα4/Dβ1/Dβ2

–

–

–

–

Dα5/Dα1/Dα2/Dβ2/Dβ3

–

–

–

–

Dα5/Dα1/Dα3/Dβ2/Dβ3

–

–

–

–

Dα5/Dα1/Dα4/Dβ2/Dβ3

–

–

–

–

Dα5/Dα1/Dβ1/Dβ2/Dβ3

–

–

–

–

Dα5/Dα2/Dα3/Dβ1/Dβ2

–

–

–

–

Dα5/Dα2/Dα4/Dβ1/Dβ2

–

–

–

–

Dα5/Dα2/Dβ1/Dβ2/Dβ3

–

–

–

–

Dα5/Dα3/Dα4/Dβ1/Dβ2

–

–

–

–

Dα5/Dα3/Dβ1/Dβ2/Dβ3

–

–

–

–

Dα5/Dα1/Dα2/Dα3/Dα4/Dβ1

–

–

–

–

Dα5/Dα1/Dα2/Dα3/Dα4/Dβ2

–

–

–

–

Dα5/Dα1/Dα2/Dα3/Dα4/Dβ3

–

–

–

–

Dα5/Dα6/Dα7/Dβ1/Dβ2/Dβ3

–

–

–

–

Dα5/Dα1/Dα2/Dα3/Dα4/Dα6/Dα7/Dβ2

–

–

–

–

Dα5/Dα1/Dα2/Dα3/Dα4/Dα6/Dα7/Dβ1/Dβ2

–

–

–

–

Dα5/Dα1/Dα2/Dα3/Dα4/Dα6/Dα7/Dβ1/Dβ2/Dβ3

–

–

–

–

  1. To illustrate the extent of radioligand binding studies undertaken, the Table lists all subunit combinations containing Dα5 that were examined in transfected Drosophila S2 cells. Binding studies were performed with [125I]-α-bungarotoxin (10 nM) and [3 H]-epibatidine (30 nM). Combinations of Drosophila nAChR subunit cDNAs were transfected in the absence or presence of RIC-3 cDNA. Data indicating presence or absence of specific binding are derived from at least 3 independent experiments.
Back to article page

BMC Neuroscience

ISSN: 1471-2202

Contact us