Figure 4

Antagonism of Dα5 nAChRs by α-bungarotoxin. A) Representative responses to acetylcholine (100 μM; black bar) are shown (left), together with block after a 10 min pre-incubation with α-bungarotoxin (100 nM; grey bar) (middle). Recovery from α-bungarotoxin block after 10 minutes is also illustrated (right). Data shown are for homomeric Dα5 nAChRs (co-expressed with CeRIC-3) but the results (complete block with full recovery) were observed for all subunit combinations that generated functional nAChRs (see Table 2). B) Data indicating the time course for recovery after a 10 min incubation with α-bungarotoxin (100 nM; illustrated by the grey bar). Data points are normalised to the maximum response prior to block by α-bungarotoxin and are means ± SEM of 3 independent experiments.