Sensory cell damage in the organ of Corti following acoustic trauma. A, Propidium iodide staining reveals malformed hair cell nuclei with a marked increase in fluorescence intensity (arrows). Uptake of propidium iodide into the nuclei indicates the loss of cell viability. Bar = 20 μm. B, Image A digitally enhanced to illustrate the weakly stained sensory cell nuclei that exhibit normal morphologies (arrows). IHC: Inner hair cells. PC: Pillar cells. OHC1, OHC2 and OHC3: The first, second, and third row of outer hair cells, respectively. C, The distribution of damaged sensory cells along the organ of Corti. Vertical lines above the bars represent one standard deviation. N: the number of cochleae examined.