Figure 1

A-F. Identification and isolation of amoeboid microglial cells (AMC) and ramified microglial cells (RMC) from the corpus callosum (CC) of 5-day old and 4-weeks old rat brain respectively. Figure A shows AMC and Figure D shows RMC stained with lectin under laser capture microscopy. These cells are laser-cut along their periphery and isolated. Figure B and E show the region of the stained tissue section wherein the cells have been removed, and figure C and F show the isolated cells collected in the cap of vial. Arrows indicate the same cells in all the three images. Scale bars: A-F 50 μm. G. Correlation plots. Correlation plots were generated in Affymetrix Expression Console 1.1 after RMA normalizing raw CEL files of AMC and RMC expression data. The color scale indicates the degree of correlation between two different samples. A value of close to 1 refers to a high correlation.