Table 1 Primers used in q-RT-PCR analysis
From: Differential effects of AdOx on gene expression in P19 embryonal carcinoma cells
Gene | Forward primer | Reverse primer |
|---|---|---|
ngn1 | CCCTGAAGACGAGGTGAAAAGT | CTTGCCATTGTATTGTCAGCCG |
mash1 | TAACTCCCAACCACTAACAGGC | TGAGGAAAGACATCAACCCAGT |
neuroD | TTCCACGTCAAGCCGC | TCGGCGGATGGTTCGT |
sox2 | CCACCAATCCCATCCA | ACCGCCTAACGTACCACT |
oct3/4 | TCACTCACATCGCCAATCA | GTAGCCTCATACTCTTCTCGTT |
nanog | GGCAGCCCTGATTCTTCTAC | CGCTTGCACTTCATCCTTT |
lin28 | CGGCCAAAAGGGAAGAACAT | CATTCCTTGGCATGATGGTCT |
fgf4 | GGCTTCGGCGGCTCTACT | AGGATTCGTAGGCGTTGTAGTTG |
utf1 | TCCTCTTACGAGCACCGACAC | GCAACGCGGTATTCAACGA |
gata6 | AACAGCAGTGGCTCTGTCCC | CTCGGGGTTGGCGTTTT |
sox17 | TGGGCCAAAGACGAACG | TTGGGGTGGTCCTGCATAT |
hand1 | CTACTTGATGGACGTGCTGG | CAACTCCCTTTTCCGCTTG |
brachyury | CCAGTCTCTGGTCTGTGAGCA | GGGGAGCCTCGAAAGAA |
pax6 | GTGAATGGGCGGAGTTATG | ACTTGGACGGGAACTGACA |
nestin | TGAGAACTCTCGCTTGCAGAC | GTATCCCAAGGAAATGCAGC |
bmp4 | CGAGCCAACACTGTGAGGA | GAGGTTGAAGAGGAAACGAAA |
cdx2 | TCAGGAGGAAAAGTGAGCTG | GGCTGTGGAGGCTGTTGTT |
gapdh | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTT |