Figure 3
Protein levels of (A) COX-2, (C) COX-1, (D) 5-LOX and (F) 12-LOX (with representative immunoblots) in control, low- and high-dose LPS rat brain. Data are ratios of optical densities of protein to β-actin, expressed as percent of control, and were compared using one-way ANOVA, mean ± SEM (n = 8). mRNA levels of (B) COX-2 and (E) 5-LOX in control, low- and high-dose LPS, measured using real time RT-PCR. Data normalized to the endogenous control (β-globulin) and relative to control level (calibrator), using the ΔΔCT method. Comparisons between LPS were made using one-way ANOVA, mean ± SEM (n = 8), *p < 0.05, **p < 0.01.