Concentration of [15N2]Arg and [13C2,15N]Gly in the medium (mmol/L) | [15N2]Arg in the cells (nmol/mg protein) | Arg in the cells (nmol/mg protein) | [15N2]Arg/Arg |
---|---|---|---|
0 | 1.8 ± 1.1 | 172.0 ± 78.2 | 0.01 ± 0.00 |
5 | 137.9 ± 71.6 NS | 145.5 ± 73.4 | 1.12 ± 0.52 |
10 | 504.0 ± 390.0 * | 178.6 ± 137.9 | 3.43 ± 1.66 |
15 | 957.4 ± 500.45 ** | 215.5 ± 132.5 | 6.74 ± 4.72 |
 | [13C2,15N]Gly in the cells (nmol/mg protein) | Gly in the cells (nmol/mg protein) | [13C2,15N]Gly/Gly |
0 | 2.8 ± 0.9 | 365.7 ± 43.2 | 0.01 ± 0.00 |
5 | 1003.8 ± 1083.3 NS | 696.3 ± 604.2 | 1.28 ± 0.49 |
10 | 2205.5 ± 2382.0 * | 893.8 ± 958.4 | 2.53 ± 0.22 |
15 | 3213.9 ± 2482.9 * | 744.8 ± 512.7 | 4.43 ± 0.85 |
 | [13C215N3]GAA in the cells (nmol/mg protein) | GAA in the cells (nmol/mg protein) | [13C215N3]GAA/GAA |
0 | 0.25 ± 0.09 | 1.46 ± 1.46 | 0.29 ± 0.14 |
5 | 10.34 ± 7.90 * | 2.37 ± 2.23 | 4.47 ± 3.97 |
10 | 17.18 ± 13.36 * | 1.88 ± 1.87 | 13.30 ± 14.32 |
15 | 21.59 ± 15.00 ** | 1.64 ± 1.11 | 18.18 ± 17.96 |
 | [13C215N3]Cr in the cells (nmol/mg protein) | Cr in the cells (nmol/mg protein) | [13C215N3]Cr/Cr |
0 | 0.10 ± 0.05 | 100.4 ± 118.7 | 0.00 ± 0.00 |
5 | 12.13 ± 12.64 * | 86.53 ± 71.65 | 0.12 ± 0.04 |
10 | 10.88 ± 8.48 ** | 59.48 ± 23.72 | 0.17 ± 0.08 |
15 | 11.00 ± 6.85 ** | 48.54 ± 10.58 | 0.21 ± 0.09 |