Figure 7

Apoptotic events during normoxic and hypoxic aestivation. TUNEL signals were evaluated in 6dAE (black), 6mAE (vertical striped), 6dMUD (diagonal striped) and 6mMUD (grey) states. At 6mAE state, an evident amount of neuronal fields containing apoptotic cells (TUNEL method, black arrow) was detected in TEL (a), Cb (c) and Te (e) with respect to the representative TUNEL control (f). In the case of 6mMUD condition, TEL (b) and Cb (d) continued to supply an evident TUNEL reaction. Variations in apoptotic cell content (g) were reported as % of apoptotic cells ± s.e.m. with respect to FW and analyzed by ANOVA and Newman Keul’s test (^ap < 0.05, ^bp < 0.01, ^cp<0.001). Scale bars (a-d) = 80 μm; (e,f) = 40 μm. For abbreviations check list.