| Â | Relative quantity HTR2CL | Relative quantity HTR2CS |
|---|
I (n = 9)
| 26.98 ± 3.66 | 51.53 ± 4.40 |
C(n = 9)
| 24.71 ± 3.21 | 44.03 ± 3.95 |
LW I (n = 4)
| 32.77 ± 3.10 | 54.37 ± 7.60 |
LW C (n = 4)
| 29.37 ± 3.70 | 51.92 ± 6.00 |
HxLW I (n = 4)
| 20.24 ± 2.30 | 45.41 ± 5.80 |
HxLW C (n = 4)
| 18.53 ± 1.0 | 38.30 ± 3.30 |
- Quantitative PCR was carried out using primer pairs HTR2CL (specific for the long isoform) and primer pair HTR2CS (both isoforms) using PSMD2 primer pairs as a control described in Quilter et al. 2008. Results from all pairs (n = 9) and from Large White pairs (n = 4) or Hampshire x Large White pairs (n = 4) in Table 5 are given as the mean ± SEM. No statistically significant differences were observed between the infanticidal and control groups within breed, but the LW results were statistically different from the HxLW results (p = 0.001) for the expression of the long isoform
