Table 1 List of Antibodies
Name | Manufacturer | Catalog#/Clone | Host | Type | Dilution | Purpose |
|---|---|---|---|---|---|---|
Neurofilament 200 kD (N52) | Sigma-Aldrich, St. Louis, MO | N0142 Clone N52 | Mouse | Mono-clonal | 1:2000 | Identify subset of nerve fibers that express NFH |
Neurofilament Heavy (NFH) | Abcam, Cambridge, MA | AB4680 | Chicken | Poly- clonal | 1:1000 | Identify subset of nerve fibers that express NFH |
Protein gene product 9.5 (PGP9.5) | Millipore, Billerica, MA | AB5898 | Guinea pig | Poly- clonal | 1:500 | Identify all nerve fibers |
Protein gene product 9.5 (PGP9.5) | UltraClone Limited, UK | Clone 31A3 | Mouse | Mono- clonal | 1:100 | Identify all nerve fibers |
Tyrosine hydroxylase (TH) | Millipore, Billerica, MA | MAB318 Clone LNC1 | Mouse | Mono- clonal | 1:100 | Identify sympathetic nerve fibers |
Von-Willebrand factor (vWF) | Dako, Denmark | A0082 | Rabbit | Poly- clonal | 1:2000 | Identify endothelial cells |
Myelin basic protein (MBP) | Millipore, Billerica, MA | MAB386 Clone 12 | Rat | Mono- clonal | 1:500 | Identify myelin associated with myelinated fibers |
Sodium channel (pan NaCh) | Rock Levinson's lab | EOIII | Rabbit | Poly- clonal | 1:100 | Identify all NaCh isoforms |
Contactin-associated protein 1 (caspr) | Elior Peles's lab | Clone 275 | Mouse | Mono- clonal | 1:500 | Labels paranode region; used to identify nodes of Ranvier |
S100 | ABR-Affinity BioReagents, Golden, CO | MA1-23594 (Clone B32.1) | Mouse | Mono- clonal | 1:500 | Identify Schwann cells |