Neuronal knockdown of both DTRH and DTH during CNS development results in normal gut fiber architecture. elavC155, pan-neuronal driver; TRHE, THK, weaker RNAi transgenes; TRHA, THA, stronger RNAi transgenes. A. Branching. B. Total varicosity number per 0.1 mm length. C. Number of large (> 1 μm2) vesicles per 0.1 mm length; D. Varicosities > 5 μm2 per 0.1 mm length. elavC155/w1118, 28 fibers from 22 guts from 4 independent experiments; elavC155/TRHE, n = 48 projecting fibers from 34 independent gut dissections; elavC155/THK, n = 40 fibers from 18 guts from 2 independent experiments; elavC155/TRHE;THK, n = 39 fibers from 28 guts from 6 independent experiments; elavC155/THA, n = 37 fibers from 21 guts from 2 independent experiments; elavC155/TRHA, n = 58 projecting fibers from 39 independent gut dissections; elavC155/THA;TRHA, n = 33 fibers from 18 guts from a single experiment. **p < 0.01, ***p < 0.001. Statistical analyses were performed using one way ANOVA and Dunnet's post-tests. Lines above the graph depict standard error of the mean.