Comparison of dye-loading protocols on stimulation by 5-HT and DOI in 5-HT
R-CHO cells. Cells plated at 16,000 cells/well two days prior to testing were either serum starved the day after plating ("Serum Starved"), serum starved only during dye loading ("No Serum during Dye Loading"), or serum replete for the entire experiment ("Serum Replete"). Calcium 4 dye reagent was added and cells were incubated for 60 min at 37°C then 60 min at RT. Cells were stimulated with a saline control medium (HBSS), 1 μM 5-HT or 1 μM DOI and peak fluorescence was recorded. n = 8 for saline and 3-4 for 5-HT and DOI.