Skip to main content
Figure 2 | BMC Neuroscience

Figure 2

From: Quantitative changes in intracellular calcium and extracellular-regulated kinase activation measured in parallel in CHO cells stably expressing serotonin (5-HT) 5-HT2A or 5-HT2Creceptors

Figure 2

Comparison of dye-loading protocols on stimulation by 5-HT and DOI in 5-HT 2A R-CHO cells. Cells plated at 16,000 cells/well two days prior to testing were either serum starved the day after plating ("Serum Starved"), serum starved only during dye loading ("No Serum during Dye Loading"), or serum replete for the entire experiment ("Serum Replete"). Calcium 4 dye reagent was added and cells were incubated for 60 min at 37°C then 60 min at RT. Cells were stimulated with a saline control medium (HBSS), 1 μM 5-HT or 1 μM DOI and peak fluorescence was recorded. n = 8 for saline and 3-4 for 5-HT and DOI.

Back to article page

BMC Neuroscience

ISSN: 1471-2202

Contact us